4/9/2023 0 Comments Waters 2777 sample manager![]() T argeted Metabolomics: applied when a small and pre-defined set of metabolites are required to be investigated and provides absolute quantification data on tens of metabolites. Two 600MHz NMR spectrometers each with a room temperature 5mm probe (Bruker).Three Ultimate3000 RSLC UHPLC systems coupled to electrospray Q Exactive Focus mass spectrometers (Thermo Scientific).Four Acquity UPLC systems with 2777c sample managers coupled to electrospray Xevo G2-XS QTof mass spectrometers (Waters).The identification of biologically or clinically significant metabolic markers (biomarkers), derived from biostatistical analysis and initial metabolite annotation, can be validated by the use of targeted assays (see below - Targeted Metabolomics). Our workflows include tools to visualise data quality, to apply biostatistics, and to identify metabolites. The data is processed, interrogated and analysed using standardised computational workflows. To ensure the quality of data meets defined acceptance criteria our fully-trained team applies standard operating procedures and quality control samples for all studies. Biocrates p400 kit applying Direct Infusion Mass Spectrometry and UPLC-MS.Reversed phase UHPLC-MS for broad coverage of lipophilic metabolites including for lipidomic studies.Hydrophilic Interaction Liquid Chromatography coupled to mass spectrometry (HILIC-MS) for a broad coverage of hydrophilic metabolites.1H NMR spectroscopy for a broad coverage of high concentration lipophilic and hydrophilic metabolites typically up to 100 metabolites are detected and quantified.We have an established analytical workflow that combines state of the art nuclear magnetic resonance (NMR) spectroscopy and ultra-high performance chromatography coupled with high resolution mass spectrometry (UHPLC-MS) to produce high quality data representing thousands of metabolites present in diverse areas of metabolism including amino acid metabolism, lipid metabolism and purine metabolism.įour specific analytical methodologies are applied: Non-Targeted Metabolomics: applied when a broad survey of different classes of metabolites is required in discovery-based studies and provides relative quantification data on thousands of metabolites. We also provide the capability to analyse other biofluids (e.g. For large-scale metabolic phenotyping studies we recommend the use of biofluids including serum, plasma and urine. Sample types: We provide the capabilities to study a diverse range of sample types. Phenome Centre Birmingham offers a complete collaborative service to provide expertise and advice to perform metabolic phenotyping studies from conception and experimental design through data acquisition to data analysis and biological interpretation. To identify metabolic biomarkers for stratification of the human population to be applied in risk stratification of developing a specific disease, in diagnosis of a disease, in defining progression or remission from a disease, and in the identification of responders and non-responders to nutritional, exercise or drug interventionsįor more information regarding how to get in touch with Phenome Centre Birmingham, please use the contact details at the bottom of this webpage.To identify molecular targets for nutritional, exercise or drug interventions.To understand molecular mechanisms associated with human ageing, disease onset and progression.Phenome Centre Birmingham conducts metabolic phenotyping studies with the following objectives: Phenome Centre Birmingham works with biomedical research groups in academia, healthcare provision (including the NHS), research institutes and industry including pharma.Ĭurrently the centre collaborates with researchers in the UK and globally to undertake metabolic phenotyping (metabolomics) in a wide range of different projects in areas including endocrinology, cancer, trauma, drug toxicity, neurodevelopmental disorders, musculoskeletal ageing, exercise and toxicology.
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